age of FFPE sections

Does this antibody work? Designing a new panel?
Antibodies, isotopes, etc. for Imaging Mass Cytometry applications
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jmarkherndon
Posts: 2
Joined: Fri Dec 14, 2018 2:21 pm

age of FFPE sections

Post by jmarkherndon » Tue Jun 18, 2019 1:59 pm

Does anyone have any thoughts about how long slides cut from FFPE blocks can be stored before staining for IMC? Should slides be stored at 4 degrees, or minus 20 degrees?
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RaulCatena
Posts: 30
Joined: Mon Nov 27, 2017 12:49 pm

Re: age of FFPE sections

Post by RaulCatena » Fri Aug 02, 2019 8:12 am

The fresher the better. The oxygen oxidizes the tissue from the moment you get the cut.

For very important tissues (TMA cuts etc) what it is done sometimes is covering with a millimeter of paraffin. I have seen also sealing in nitrogen bags.
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