PD-1 antibody

Does this antibody work? Designing a new panel?
Antibodies, isotopes, etc. for Imaging Mass Cytometry applications
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ssivajothi
Posts: 33
Joined: Thu Jan 11, 2018 9:00 pm

PD-1 antibody

Post by ssivajothi » Wed Dec 11, 2019 9:38 pm

Hi all,

Does anyone know of a clone of PD-1 that works well for acetone fixed frozen sections (human)?

Thank you!
Santhosh
eliom
Posts: 1
Joined: Mon Mar 09, 2020 3:07 pm

Re: PD-1 antibody

Post by eliom » Mon Mar 09, 2020 3:10 pm

Hi ssivajothi,

I'm also looking for PD-1 antibody to use on FFPE sections from human duodenum. I tried the one from Fluidigm, but I get a lot of background. Did you find something else that seems to work on human FFPE sections?

Many thanks!
Elio
HenrikeSal
Posts: 1
Joined: Mon Oct 21, 2019 11:35 am

Re: PD-1 antibody

Post by HenrikeSal » Sun May 10, 2020 2:13 pm

Hi Elio and Santhosh,

I've had problems with the PD-1 antibody from fluidigm on human FFPE liver sections as well. I have now tried the D4W2J clone from cell signaling and the NAT105 clone from BioLegend which both work better. The signal intensities are still quite low but at least seem to be specific. I hope this helps.
I haven't tried anything on frozen sections though, sorry.

Best,
Henrike
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